Assessment of the characterization antimicrobial and green synthesis of magnesium oxide nanoparticles using Psydium guajava - in-vitro analysi

Aim: To assess the characterization, antimicrobial activity of green synthesised magnesium oxide nanoparticles using Psydium guajava leaves. Materials And Methods: leaves were dried and ground, which 2 g powder was used for study.the then dissolved in 100ml distilled water. 1 mg chloride salt 20 ml Both solutions mixed subjected to centrifuge. After preparation nanoparticle, it further investigation like SEM analysis, EDAX, UV-VIs, cytotoxicity anti microbial tests. The investigated a brine shrimps bioassay.The number surviving naupli cells manually counted under dissection microscope. antibacterial examined against four bacterial strains- E.coli, E.fecalis,S.aureus,P.aurogenosa by agar well diffusion method .The inoculated plates incubated overnight (for about 24 hours at 37 degree celsius) inhibition zone around nanoparticle solution calculated. tests carried standard antibiotic results tabulated. Results: analysis showed particle size 70 nm UV -Visible spectroscopy peak 320nm confirming presence that is prepared guajava. Cytotoxicty revealed 83% shrimp survived effective two micro-organisms -S.aureus E.fecalis their nearly equal organisms incubated. Conclusion: synthesized found have good acceptable anti-microbial especially most commonly harbouring micro-organism(S.aureus E.faecalis) .Hence they can be extended use soft tissue healing.